An edible fungi Pleurotus ostreatus inhibits adipogenesis via suppressing expression of PPAR γ and C/EBP α in 3T3-L1 cells: In vitro validation of gene knock out of RNAs in PPAR γ using CRISPR spcas9 – Bindhu J. & Al.
Experiment: in vitro (cell cultures)
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Oyster mushroom (Pleurotus ostreatus) can inhibit adipogenesis by suppressing the expression of PPAR γ and C/EBP α in 3T3-L1 cells.

Obesity, characterized by excessive fat accumulation, is a major risk factor for cardiovascular diseases, diabetes, and certain cancers. Oyster mushrooms possess anti-obesity properties. This study tested their effects on adipogenesis (fat cell formation) using 3T3-L1 cells, which differentiate into fat cells.

Cells were cultured with varying concentrations of oyster mushroom extract. Results showed the extract inhibited adipogenesis by suppressing PPAR γ (a nuclear receptor regulating lipid metabolism) and C/EBP α (a transcription factor stimulating fat cell differentiation). This led to reduced fat cell formation and lower lipid levels in cells.

These findings suggest oyster mushrooms may act as anti-obesity agents. Further studies are needed to confirm these results and determine optimal dosages.

Pleurotus Ostreatus Ameliorates Obesity by Modulating the Gut Microbiota in Obese Mice Induced by High-Fat Diet – Yanzhou Hu & Al.
Experiment: in vivo (Mus musculus – mouse)
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Oyster mushrooms (Pleurotus ostreatus) may prevent obesity by modulating gut microbiota composition and function.

In obese mice fed a high-fat diet, oyster mushroom administration led to significant weight loss, altered gut microbiota composition, and improved microbial function compared to controls.

Key findings:

• Increased beneficial bacteria (e.g., Oscillospira, Lactobacillus, Bifidobacterium), which aid lipid/carbohydrate metabolism and bile acid regulation.

• Reduced harmful bacteria (e.g., Bacteroides, Roseburia), which promote inflammation and fat storage.

This study highlights oyster mushrooms as a potential natural treatment for obesity. Further research is required for confirmation.